Elimination of suction effect in interfacing microchip electrophoresis with inductively coupled plasma mass spectrometry using porous monolithic plugs

Abstract

A suction-free interfacing method was developed for microchip electrophoresis hyphenated with inductively coupled plasma mass spectrometry (MCE-ICP-MS). The hyphenated system was composed of a microchip, a demountable capillary microflow nebulizer (d-CMN) combined with a heated single pass spray chamber, a negative pressure sampling device, a high voltage power supply, a syringe pump and an ICP-MS. To eliminate the nebulizer suction generated by the pneumatic nebulizer and to ensure that the makeup solution flowed into the nebulizer, two porous polymer plugs were fabricated in the microchip. As a result, reasonably true electropherograms were obtained when compared to the CE separation performed in the traditional MCE-ICP-MS mode without porous polymer plugs. Electrophoretic separation of I⁻ and IO₃⁻ was achieved within 25 s in a microchip with an effective separation length of only 15 mm at an electric field of 857 V cm⁻¹ using 10 mmol L⁻¹ borate (pH 9.2) as the running buffer. A resolution of 1.3 was obtained and the absolute detection limits for I⁻ and IO₃⁻ were 0.12 and 0.13 fg, respectively. The precisions (RSD, n = 10) of the migration time and peak height for I⁻ and IO₃⁻ were in the range of 1.1–1.6% and 2.5–2.8%, respectively. Two table salt samples were analyzed by an external calibration method. The iodate contents were in accordance with their labeled values. The recoveries of I⁻ and IO₃⁻ in the table salt samples were in the range of 92–105%.