Issue 15, 2012

Generating contrast in hyperpolarized 13C MRI using ligand–receptor interactions

Abstract

We report the imaging of β-cyclodextrin–benzoic acid binding at 14T using hyperpolarized 13C magnetic resonance (MR). Benzoic acid was polarized using a dynamic nuclear polarization (DNP) approach and combined with β-cyclodextrin in aqueous solution. As anticipated, decreases in the spin–lattice relaxation constant (T1) were observed with decreases in the ligand–receptor ratio. The calculated log K was approximately 1.7, similar to previously reported binding constants. Hyperpolarized [1-13C] benzoic acid was used to interrogate solutions of variable β-cyclodextrin concentrations, with the mixtures imaged at 14T using a 3D frequency-selective MR sequence. Differences in β-cyclodextrin concentration were easily visualized. These results suggest that hyperpolarized 13C MR could be used in vivo to determine the presence and density of receptors for a given ligand–receptor pair.

Graphical abstract: Generating contrast in hyperpolarized 13C MRI using ligand–receptor interactions

Supplementary files

Article information

Article type
Communication
Submitted
27 Mar 2012
Accepted
03 May 2012
First published
04 May 2012

Analyst, 2012,137, 3427-3429

Generating contrast in hyperpolarized 13C MRI using ligand–receptor interactions

K. R. Keshari, J. Kurhanewicz, J. M. Macdonald and D. M. Wilson, Analyst, 2012, 137, 3427 DOI: 10.1039/C2AN35406C

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