Issue 1, 2012
From the journal:

Analytical Methods

A facile transport assay for H+ coupled membrane transport using fluorescence probes†

Wanjun Lan,a   Hongliu Ren,a   Yu Pang,a   Chuseng Huang,a   Yufang Xu,a   Robert J. Brooker*b  and  Jingyan Zhang*a  

* Corresponding authors

a State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai
E-mail: jyzhang@ecust.edu.cn
Fax: +86-021-64253846

b Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA
E-mail: brook005@umn.edu

Abstract

A facile activity assay for an H+-coupled transporter using florescent probes was developed with an H+-coupled manganese transporter (MntH) as a model. Making use of coupled-proton transport, the transport activity (H+/Mn2+ cotransport) can be directly determined via fluorescence intensity changes of the probe, 5-(and-6)-carboxyfluorescein (5(6)-FAM). The approach of using highly sensitive fluorescence probes provides a more simple and convenient assay method for the determination of proton-coupled metal-ion uptake by transporters.

Graphical abstract: A facile transport assay for H+ coupled membrane transport using fluorescence probes

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C1AY05549F
Article type
Communication
Submitted
02 Sep 2011
Accepted
02 Nov 2011
First published
14 Nov 2011

Anal. Methods, 2012,4, 44-46

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A facile transport assay for H+ coupled membrane transport using fluorescence probes

W. Lan, H. Ren, Y. Pang, C. Huang, Y. Xu, R. J. Brooker and J. Zhang, Anal. Methods, 2012, 4, 44 DOI: 10.1039/C1AY05549F

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