Issue 4, 2012
From the journal:

Chemical Communications

Design of a multinuclear Zn(ii) complex as a new molecular probe for fluorescence imaging of His-tag fused proteins†

Sho-hei Fujishima,a   Hiroshi Nonaka,a   Sho-hei Uchinomiya,a   Yoshiyuki Alex. Kawase,a   Akio Ojidab  and  Itaru Hamachi*a  

* Corresponding authors

a Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto, Japan
E-mail: ihamachi@sbchem.kyoto-u.ac.jp

b Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka, Japan

Abstract

A Zn(II) complex (Zn(II)–Ida) was designed as the new fluorescent probe for His-tag fused proteins. Thanks to the tight binding ability to histidine-rich sequences and bright fluorescence property of the Cy5-appended Zn(II)–Ida probes, selective and clear fluorescent imaging of the His-tag fused G-protein coupled receptors on live cell surfaces was carried out.

Graphical abstract: Design of a multinuclear Zn(ii) complex as a new molecular probe for fluorescence imaging of His-tag fused proteins

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Article information

DOI
https://doi.org/10.1039/C1CC16263B
Article type
Communication
Submitted
08 Oct 2011
Accepted
11 Nov 2011
First published
15 Nov 2011

Chem. Commun., 2012,48, 594-596

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Design of a multinuclear Zn(II) complex as a new molecular probe for fluorescence imaging of His-tag fused proteins

S. Fujishima, H. Nonaka, S. Uchinomiya, Y. Alex. Kawase, A. Ojida and I. Hamachi, Chem. Commun., 2012, 48, 594 DOI: 10.1039/C1CC16263B

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