Issue 11, 2012
From the journal:

Chemical Communications

A surface-based mass spectrometry method for screening glycosidase specificity in environmental samples†

Ana Beloqui,‡a   Antonio Sanchez-Ruiz,‡a   Manuel Martin-Lomasab  and  Niels-Christian Reichardt*ab  

* Corresponding authors

a Biofunctional Nanomaterials Department, CICbiomaGUNE, Paseo Miramon 182, 20009 San Sebastian, Spain

b CIBER-BBN, 20009 San Sebastian, Spain
E-mail: nreichardt@cicbiomagune.es

Abstract

A new surface-based MALDI-Tof-MS glycosyl hydrolase assay has been developed in which lipid-tagged oligosaccharides, representing defined fragments of major plant cell wall polysaccharides, are immobilized via hydrophobic interactions on an alkylthiol functionalised gold sample plate and employed in the functional screening of several purified enzymes, environmental samples and saliva.

Graphical abstract: A surface-based mass spectrometry method for screening glycosidase specificity in environmental samples

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Article information

DOI
https://doi.org/10.1039/C2CC16537F
Article type
Communication
Submitted
20 Oct 2011
Accepted
02 Dec 2011
First published
06 Dec 2011

Chem. Commun., 2012,48, 1701-1703

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A surface-based mass spectrometry method for screening glycosidase specificity in environmental samples

A. Beloqui, A. Sanchez-Ruiz, M. Martin-Lomas and N. Reichardt, Chem. Commun., 2012, 48, 1701 DOI: 10.1039/C2CC16537F

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