Issue 21, 2012
From the journal:

Chemical Communications

Thiol–ene reaction: a versatile tool in site-specific labelling of proteins with chemically inert tags for paramagnetic NMR†

Qing-Feng Li,a   Yin Yang,a   Ansis Maleckis,b   Gottfried Otting*b  and  Xun-Cheng Su*ab  

* Corresponding authors

a State-Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin, China
E-mail: xunchengsu@nankai.edu.cn

b Research School of Chemistry, Australian National University, Canberra, Australia
E-mail: go@rsc.anu.edu.au

Abstract

Site-specific tagging of proteins with paramagnetic lanthanides generates valuable long-range structure restraints for structural biology by NMR spectroscopy. We show that the thiol–ene addition reaction offers a powerful tool for tagging proteins in a chemically stable manner with very small lanthanide tags.

Graphical abstract: Thiol–ene reaction: a versatile tool in site-specific labelling of proteins with chemically inert tags for paramagnetic NMR

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Article information

DOI
https://doi.org/10.1039/C2CC17900H
Article type
Communication
Submitted
18 Dec 2011
Accepted
19 Jan 2012
First published
19 Jan 2012

Chem. Commun., 2012,48, 2704-2706

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Thiol–ene reaction: a versatile tool in site-specific labelling of proteins with chemically inert tags for paramagnetic NMR

Q. Li, Y. Yang, A. Maleckis, G. Otting and X. Su, Chem. Commun., 2012, 48, 2704 DOI: 10.1039/C2CC17900H

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