Issue 40, 2012
From the journal:

Chemical Communications

A TEMPO-conjugated fluorescent probe for monitoring mitochondrial redox reactions†

Shota Hirosawa,a   Satoshi Araib  and  Shinji Takeoka*a  

* Corresponding authors

a Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University (TWIns), Tokyo 162-8480, Japan
E-mail: takeoka@waseda.jp
Tel: +81-3-5369-7324

b Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, Tokyo 162-0041, Japan

Abstract

We report a mitochondrial targeted redox probe (MitoRP) that comprises a nitroxide radical (TEMPO) moiety and coumarin 343. Using isolated mitochondria in the presence/absence of substrates and inhibitors of oxidative phosphorylation, we demonstrated that MitoRP is a useful probe to monitor the electron flow associated with complex I.

Graphical abstract: A TEMPO-conjugated fluorescent probe for monitoring mitochondrial redox reactions

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Article information

DOI
https://doi.org/10.1039/C2CC30603D
Article type
Communication
Submitted
27 Jan 2012
Accepted
28 Mar 2012
First published
29 Mar 2012

Chem. Commun., 2012,48, 4845-4847

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A TEMPO-conjugated fluorescent probe for monitoring mitochondrial redox reactions

S. Hirosawa, S. Arai and S. Takeoka, Chem. Commun., 2012, 48, 4845 DOI: 10.1039/C2CC30603D

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