Issue 44, 2012
From the journal:

Chemical Communications

Immobilisation of quantum dots by bio-orthogonal PCR amplification and labelling for direct gene detection and quantitation†

Quan Tian,a   Winnie Wong,b   Yang Xu,a   Yinthai Chan,a   Han Kiat Ho,b   Giorgia Pastorinb  and  Wee Han Ang*a  

* Corresponding authors

a Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore
E-mail: chmawh@nus.edu.sg
Fax: +65 6779 1691
Tel: +65 6516 5131

b Department of Pharmacy, National University of Singapore, 3 Science Drive 3, Singapore

Abstract

A sensitive and versatile detection scheme based on quantum dot immobilisation on a solid support through bio-orthogonal PCR amplification and labelling has been developed for detection and quantification of gene targets in complex DNA mixtures.

Graphical abstract: Immobilisation of quantum dots by bio-orthogonal PCR amplification and labelling for direct gene detection and quantitation

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Article information

DOI
https://doi.org/10.1039/C2CC30680H
Article type
Communication
Submitted
31 Jan 2012
Accepted
06 Apr 2012
First published
10 Apr 2012

Chem. Commun., 2012,48, 5467-5469

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Immobilisation of quantum dots by bio-orthogonal PCR amplification and labelling for direct gene detection and quantitation

Q. Tian, W. Wong, Y. Xu, Y. Chan, H. K. Ho, G. Pastorin and W. H. Ang, Chem. Commun., 2012, 48, 5467 DOI: 10.1039/C2CC30680H

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