Microstructured fluorescent biosensor based on energy migration for selective sensing of metalloprotein†
Abstract
Fluorescent microspheres consisting of a conjugated phenylenevinylene 3B2B doped with a fluorenone derivative DSFO were prepared by a reprecipitation method. The DSFO-doped 3B2B microspheres (DMPs) exhibited significantly reduced fluorescence from 3B2B (donor) and strongly enhanced emission from DSFO (acceptor), which is highly sensitive to the concentration of