Issue 52, 2012

A selective Seoul-Fluor-based bioprobe, SfBP, for vaccinia H1-related phosphatase—a dual-specific proteintyrosine phosphatase

Abstract

We report a Seoul-Fluor-based bioprobe, SfBP, for selective monitoring of protein tyrosine phosphatases (PTPs). A rational design based on the structures at the active site of dual-specific PTPs can enable SfBP to selectively monitor the activity of these PTPs with a 93-fold change in brightness. Moreover, screening results of SfBP against 30 classical PTPs and 35 dual-specific PTPs show that it is selective toward vaccinia H1-related (VHR) phosphatase, a dual-specific PTP (DUSP-3).

Graphical abstract: A selective Seoul-Fluor-based bioprobe, SfBP, for vaccinia H1-related phosphatase—a dual-specific protein tyrosine phosphatase

Supplementary files

Article information

Article type
Communication
Submitted
07 Jan 2012
Accepted
04 May 2012
First published
04 May 2012

Chem. Commun., 2012,48, 6553-6555

A selective Seoul-Fluor-based bioprobe, SfBP, for vaccinia H1-related phosphatase—a dual-specific protein tyrosine phosphatase

M. S. Jeong, E. Kim, H. J. Kang, E. J. Choi, A. R. Cho, S. J. Chung and S. B. Park, Chem. Commun., 2012, 48, 6553 DOI: 10.1039/C2CC31377D

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