Issue 53, 2012
From the journal:

Chemical Communications

Development of a fluorescent chalcone library and its application in the discovery of a mouse embryonic stem cell probe†

Sung-Chan Lee,‡a   Nam-Young Kang,‡a   Sung-Jin Park,a   Seong-Wook Yun,a   Yogeswari Chandrana  and  Young-Tae Chang*ab  

* Corresponding authors

a Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore 138667

b Department of Chemistry, National University of Singapore, MedChem Program of Life Sciences Institute, National University of Singapore, 3 Science Drive 3, Singapore 117543
E-mail: chmcyt@nus.edu.sg

Abstract

We report the first fluorescent diamino-chalcone library and its application in the discovery of a mouse embryonic stem cell (mESC) probe. CDg4, a novel green fluorescent mESC probe was discovered through a high-content image based screening of 160 members of the chalcone library. Interestingly, the molecular binding target of CDg4 was identified as the glycogen of the stem cell colony surface, rather than a conventional protein target from an intracellular source.

Graphical abstract: Development of a fluorescent chalcone library and its application in the discovery of a mouse embryonic stem cell probe

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Article information

DOI
https://doi.org/10.1039/C2CC31662E
Article type
Communication
Submitted
06 Mar 2012
Accepted
11 May 2012
First published
29 May 2012

Chem. Commun., 2012,48, 6681-6683

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Development of a fluorescent chalcone library and its application in the discovery of a mouse embryonic stem cell probe

S. Lee, N. Kang, S. Park, S. Yun, Y. Chandran and Y. Chang, Chem. Commun., 2012, 48, 6681 DOI: 10.1039/C2CC31662E

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