Issue 67, 2012
From the journal:

Chemical Communications

Residue choice defines efficiency and influence of bioorthogonal protein modification via genetically encoded strain promoted Click chemistry†

Samuel C. Reddington,ab   Eric M. Tippmann*b  and  D. Dafydd Jones*a  

* Corresponding authors

a School of Biosciences, Cardiff University, Cardiff, UK
E-mail: jonesdd@cardiff.ac.uk
Tel: +44 (0)2920 874290

b School of Chemistry, Cardiff University, Cardiff, UK
E-mail: tippmann@cardiff.ac.uk
Tel: +44 (0)2920 870452

Abstract

GFP and a FRET compatible dye were used to assess the influence of genetically encoded aryl azide positioning on Click chemistry-based protein conjugation. While modification efficiency of the sampled mutants using a strain promoted reaction varied by as much as ∼10 fold, there was no simple correlation with accessibility of the aryl azide on GFP's surface. One labeled GFP mutant (Gln204AzPhe) exhibited high efficiency FRET (∼90%) and an unprecedented pseudo-Stokes shift of 126 nm.

Graphical abstract: Residue choice defines efficiency and influence of bioorthogonal protein modification via genetically encoded strain promoted Click chemistry

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Article information

DOI
https://doi.org/10.1039/C2CC31887C
Article type
Communication
Submitted
14 Mar 2012
Accepted
04 Jul 2012
First published
04 Jul 2012

Chem. Commun., 2012,48, 8419-8421

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Residue choice defines efficiency and influence of bioorthogonal protein modification via genetically encoded strain promoted Click chemistry

S. C. Reddington, E. M. Tippmann and D. Dafydd Jones, Chem. Commun., 2012, 48, 8419 DOI: 10.1039/C2CC31887C

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