Issue 89, 2012
From the journal:

Chemical Communications

A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge†‡§

Salifu Seidu-Larry,a   Bettina Krieg,a   Markus Hirsch,a   Mark Helma  and  Olwen Domingo*a  

* Corresponding authors

a Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, 5 Staudinger Weg, Mainz, Germany
E-mail: dominol@uni-mainz.de
Fax: +49 6131 39 20373
Tel: +49 6131 39 24352

Abstract

A propargyl containing guanosine phosphoramidite was synthesized and incorporated into siRNA, enabling click-ligation with an azido fluorophore onto the nucleobase sugar edge. Duplex stability was not affected by labeling at this new site, which allowed deconvolution of the effects of label, structure and attachment site on RNAi activity.

Graphical abstract: A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge

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Article information

DOI
https://doi.org/10.1039/C2CC34015A
Article type
Communication
Submitted
05 Jun 2012
Accepted
17 Sep 2012
First published
18 Sep 2012
This article is Open Access

Chem. Commun., 2012,48, 11014-11016

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A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge

S. Seidu-Larry, B. Krieg, M. Hirsch, M. Helm and O. Domingo, Chem. Commun., 2012, 48, 11014 DOI: 10.1039/C2CC34015A

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