Issue 65, 2012
From the journal:

Chemical Communications

Templated chemistry for monitoring damage and repair directly in duplex DNA†‡§

Seoung Ho Lee,a   Shenliang Wanga  and  Eric T. Kool*a  

* Corresponding authors

a Department of Chemistry, Stanford University, Stanford, California 94305, USA
E-mail: kool@stanford.edu
Fax: +1 650 725 0259
Tel: +1 650 724 4741

Abstract

We report the fluorogenic detection of the product of base excision repair (an abasic site) in a specific sequence of duplex DNA. This is achieved by DNA-templated chemistry, employing triple helix-forming probes that contain unnatural nucleobases designed to selectively recognize the site of a missing base. Light-up signals of up to 36-fold were documented, and probes could be used to monitor enzymatic removal of a damaged base.

Graphical abstract: Templated chemistry for monitoring damage and repair directly in duplex DNA

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Article information

DOI
https://doi.org/10.1039/C2CC34060G
Article type
Communication
Submitted
06 Jun 2012
Accepted
27 Jun 2012
First published
28 Jun 2012

Chem. Commun., 2012,48, 8069-8071

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Templated chemistry for monitoring damage and repair directly in duplex DNA

S. H. Lee, S. Wang and E. T. Kool, Chem. Commun., 2012, 48, 8069 DOI: 10.1039/C2CC34060G

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