Issue 9, 2012
From the journal:

Lab on a Chip

Walking nanothermometers: spatiotemporal temperature measurement of transported acidic organelles in single living cells†

Kotaro Oyama,‡a   Masao Takabayashi,‡b   Yoshiaki Takei,b   Satoshi Arai,c   Shinji Takeoka,bd   Shin'ichi Ishiwata*ad  and  Madoka Suzuki*d  

* Corresponding authors

a Department of Physics, School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan
E-mail: ishiwata@waseda.jp
Fax: +81-3-5286-3437
Tel: +81-3-5286-3437

b Department of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan

c Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, Tokyo, Japan

d Waseda Bioscience Research Institute in Singapore (WABIOS), Waseda University, 11 Biopolis Way, #05-01/02 Helios, Singapore 138667, Singapore
E-mail: suzu_mado@aoni.waseda.jp
Fax: +65-6478-9416
Tel: +65-6478-9721

Abstract

We fabricated fluorescent nanoparticles which monitor temperature changes without sensitivity to pH (4–10) and ionic strength (0–500 mM). The nanothermometers spontaneously enter living HeLa cells via endocytosis, enclosed in acidic organelles, i.e., endosome/lysosome, and then transported along microtubules in a temperature-dependent manner, working as “walking nanothermometers”.

Graphical abstract: Walking nanothermometers: spatiotemporal temperature measurement of transported acidic organelles in single living cells

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Article information

DOI
https://doi.org/10.1039/C2LC00014H
Article type
Communication
Submitted
04 Jan 2012
Accepted
05 Mar 2012
First published
08 Mar 2012

Lab Chip, 2012,12, 1591-1593

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Walking nanothermometers: spatiotemporal temperature measurement of transported acidic organelles in single living cells

K. Oyama, M. Takabayashi, Y. Takei, S. Arai, S. Takeoka, S. Ishiwata and M. Suzuki, Lab Chip, 2012, 12, 1591 DOI: 10.1039/C2LC00014H

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