Issue 12, 2012
From the journal:

Organic & Biomolecular Chemistry

Deracemization of unnatural amino acid: homoalanine using d-amino acid oxidase and ω-transaminase†

Young-Man Seo,a   Sam Mathew,a   Han-Seop Bea,a   Yong-Ho Khang,a   Sang-Hyeup Lee,b   Byung-Gee Kimc  and  Hyungdon Yun*a  

* Corresponding authors

a School of Biotechnology, Yeungnam University, Gyeongbuk, South Korea
E-mail: hyungdon@ynu.ac.kr
Fax: +82-53-810-4769

b Department of Life Chemistry, Catholic University of Daegu, Gyeongbuk, South Korea

c School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, South Korea

Abstract

A deracemization method was developed to generate optically pure L-homoalanine from racemic homoalanine using D-amino acid oxidase and ω-transaminase. A whole cell reaction using a biphasic system converted 500 mM racemic homoalanine to 485 mM L-homoalanine (>99% ee).

Graphical abstract: Deracemization of unnatural amino acid: homoalanine using d-amino acid oxidase and ω-transaminase

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Article information

DOI
https://doi.org/10.1039/C2OB07161D
Article type
Paper
Submitted
22 Dec 2011
Accepted
26 Jan 2012
First published
20 Feb 2012

Org. Biomol. Chem., 2012,10, 2482-2485

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Deracemization of unnatural amino acid: homoalanine using D-amino acid oxidase and ω-transaminase

Y. Seo, S. Mathew, H. Bea, Y. Khang, S. Lee, B. Kim and H. Yun, Org. Biomol. Chem., 2012, 10, 2482 DOI: 10.1039/C2OB07161D

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