Issue 17, 2012
From the journal:

Organic & Biomolecular Chemistry

Fluorescence-based active site probes for profiling deubiquitinating enzymes†

Joanna F. McGouran,a   Holger B. Kramer,b   Mukram M. Mackeen,a   Katalin di Gleria,a   Mikael Altunac  and  Benedikt M. Kessler*a  

* Corresponding authors

a Nuffield Department of Medicine, University of Oxford, Henry Welcome Building for Molecular Physiology, Roosevelt Drive, Oxford, UK
E-mail: bmk@ccmp.ox.ac.uk
Fax: +01865287787
Tel: +08165 287799

b Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford, UK

c Present address: Unit of Translational Medicine and Chemical Biology, Dept. of Biophysics and Biochemistry, Karolinska Institutet, 17177 Stockholm, Sweden

Abstract

Novel ubiquitin-based active site probes including a fluorescent tag have been developed and evaluated. A new, functionalizable electrophilic trap is utilized allowing for late stage diversification of the probe. Attachment of fluorescent dyes allowed direct detection of endogenous deubiquitinating enzyme (DUB) activities in cell extracts by in-gel fluorescence imaging.

Graphical abstract: Fluorescence-based active site probes for profiling deubiquitinating enzymes

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Article information

DOI
https://doi.org/10.1039/C2OB25258A
Article type
Communication
Submitted
03 Feb 2012
Accepted
07 Mar 2012
First published
28 Mar 2012

Org. Biomol. Chem., 2012,10, 3379-3383

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Fluorescence-based active site probes for profiling deubiquitinating enzymes

J. F. McGouran, H. B. Kramer, M. M. Mackeen, K. di Gleria, M. Altun and B. M. Kessler, Org. Biomol. Chem., 2012, 10, 3379 DOI: 10.1039/C2OB25258A

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