A highly sensitive fluorescent probe based on simple pyrazoline for Zn2+ in living neuron cells

Zhe Zhang; Fang-Wu Wang; Sheng-Qing Wang; Fei Ge; Bao-Xiang Zhao; Jun-Ying Miao

doi:10.1039/C2OB26375K

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A highly sensitive fluorescent probe based on simple pyrazoline for Zn²⁺ in living neuron cells†

Zhe Zhang,^a Fang-Wu Wang,^b Sheng-Qing Wang,^a Fei Ge,^a Bao-Xiang Zhao*^a and Jun-Ying Miao*^b

Author affiliations

* Corresponding authors

^a Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P. R. China
E-mail: bxzhao@sdu.edu.cn

^b Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, P. R. China
E-mail: miaojy@sdu.edu.cn

Abstract

We develop a pyrazoline-based fluorescent sensor for biological Zn²⁺ detection. The sensor shows good binding selectivity for Zn²⁺ over competing metal with 40-fold fluorescence enhancement in response to Zn²⁺. The new probe is cell-permeable and can be used to detect intracellular zinc ions in living neuron cells.

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Article information

DOI: https://doi.org/10.1039/C2OB26375K
Article type: Paper
Submitted: 17 Jul 2012
Accepted: 10 Sep 2012
First published: 12 Sep 2012

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Org. Biomol. Chem., 2012,10, 8640-8644

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A highly sensitive fluorescent probe based on simple pyrazoline for Zn²⁺ in living neuron cells

Z. Zhang, F. Wang, S. Wang, F. Ge, B. Zhao and J. Miao, Org. Biomol. Chem., 2012, 10, 8640 DOI: 10.1039/C2OB26375K

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Organic & Biomolecular Chemistry