RT-isoPCR provides multiplex amplification of mRNA targets using a first-stage multiplex RT-PCR reaction with subsequent isothermal amplification for individual target loci detection. We demonstrate detection of 24 mRNA targets with high specificity and sensitivity without compromising sample variation or introducing biases between targets.
M. J. Søe and P. Warthoe, Analyst, 2013, 138, 5871 DOI: 10.1039/C3AN00803G
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