Issue 17, 2013
From the journal:

Analyst

An enzyme-free and label-free assay for copper(ii) ion detection based on self-assembled DNA concatamers and Sybr Green I†

Chenchen Ge,‡ab   Junhua Chen,‡a   Wei Wu,a   Zhiyuan Fang,a   Lingbo Chen,ac   Qi Liu,a   Lin Wang,a   Xuerong Xinga  and  Lingwen Zeng*a  

* Corresponding authors

a Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
E-mail: zeng6@yahoo.com
Fax: +86 20 32015245
Tel: +86 20 32015312

b School of Life Sciences, University of Science and Technology of China, Hefei, China

c School of Life Sciences, Anhui University, Hefei 230601, P.R. China

Abstract

An enzyme-free and label-free fluorescence turn on biosensor for amplified copper(II) ion (Cu2+) detection has been constructed based on self-assembled DNA concatamers and Sybr Green I. This assay is simple, inexpensive and sensitive, enabling quantitative detection of as low as 12.8 pM Cu2+.

Graphical abstract: An enzyme-free and label-free assay for copper(ii) ion detection based on self-assembled DNA concatamers and Sybr Green I

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C3AN00973D
Article type
Communication
Submitted
15 May 2013
Accepted
09 Jun 2013
First published
10 Jun 2013

Analyst, 2013,138, 4737-4740

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An enzyme-free and label-free assay for copper(II) ion detection based on self-assembled DNA concatamers and Sybr Green I

C. Ge, J. Chen, W. Wu, Z. Fang, L. Chen, Q. Liu, L. Wang, X. Xing and L. Zeng, Analyst, 2013, 138, 4737 DOI: 10.1039/C3AN00973D

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