Issue 6, 2013

Suspended microtubules demonstrate high sensitivity and low experimental variability in kinesin bead assay

Abstract

Microtubule (MT) based intraneuronal transport deficiency is directly linked to neurodegeneration. Hence, the development of a reliable and sensitive in vitro approach permitting efficient analysis of MT-based transport is essential for our understanding of the underlying molecular mechanisms that may lead to novel therapeutic approaches for treating neurodegenerative diseases. Here, based on previously developed reconstructed MT–kinesin assay, we propose its “suspended” modification that shows higher sensitivity and lower experimental variability.

Graphical abstract: Suspended microtubules demonstrate high sensitivity and low experimental variability in kinesin bead assay

Article information

Article type
Communication
Submitted
08 Aug 2012
Accepted
24 Jan 2013
First published
24 Jan 2013

Analyst, 2013,138, 1653-1656

Suspended microtubules demonstrate high sensitivity and low experimental variability in kinesin bead assay

M. C. Tarhan, Y. Orazov, R. Yokokawa, S. L. Karsten and H. Fujita, Analyst, 2013, 138, 1653 DOI: 10.1039/C3AN36545J

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