Issue 11, 2013

Integrated label-free silicon nanowire sensor arrays for (bio)chemical analysis

Abstract

We present a label-free (bio)chemical analysis platform that uses all-electrical silicon nanowire sensor arrays integrated with a small volume microfluidic flow-cell for real-time (bio)chemical analysis and detection. The integrated sensing platform contains an automated multi-sample injection system that eliminates erroneous sensor responses from sample switching due to flow rate fluctuations and provides precise sample volumes down to 10 nl. Biochemical sensing is demonstrated with real-time 15-mer DNA–PNA (peptide nucleic acid) duplex hybridization measurements from different sample concentrations in a low ionic strength, and the equilibrium dissociation constant KD ≈ 140 nM has been extracted from the experimental data using the first order Langmuir binding model. Chemical sensing is demonstrated with pH measurements from different injected samples in flow that have sensitivities consistent with the gate-oxide materials. A differential sensor measurement configuration results in a 30× reduction in sensor drift. The integrated label-free analysis platform is suitable for a wide range of small volume chemical and biochemical analyses.

Graphical abstract: Integrated label-free silicon nanowire sensor arrays for (bio)chemical analysis

Supplementary files

Article information

Article type
Paper
Submitted
31 Oct 2012
Accepted
28 Mar 2013
First published
28 Mar 2013
This article is Open Access
Creative Commons BY license

Analyst, 2013,138, 3221-3229

Integrated label-free silicon nanowire sensor arrays for (bio)chemical analysis

A. De, J. van Nieuwkasteele, E. T. Carlen and A. van den Berg, Analyst, 2013, 138, 3221 DOI: 10.1039/C3AN36586G

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