Issue 7, 2013

Label-free, disposable fiber-optic biosensors for DNA hybridization detection

Abstract

A novel and highly sensitive fiber-optic DNA sensor based on a thin-core fiber modal interferometer (TCFMI) is demonstrated by using a layer-by-layer (LbL) self-assembly technology. Poly(ethylenimine) (PEI), poly(acrylic acid) (PAA) and single-stranded DNA (ssDNA) were used for the preparation of a polyelectrolyte multilayer film for DNA detection. The film thickness was measured through a surface profilometer. The surface morphologies of (PEI/PAA)4, (PEI/PAA)4.5 and (PEI/PAA)4(PEI/DNA)1 multilayer films were characterized by atomic force microscopy. The fabricated DNA sensors were tested with different types of target ssDNA solutions with a concentration of 1 μM. The results show that the sensitivity of the TCFMI-based ssDNA sensor is 0.27 nm/matched-base at the concentration of 1 μM and can even distinguish the number of matched bases of ssDNA chains.

Graphical abstract: Label-free, disposable fiber-optic biosensors for DNA hybridization detection

Supplementary files

Additions and corrections

Article information

Article type
Paper
Submitted
24 Aug 2012
Accepted
15 Jan 2013
First published
16 Jan 2013

Analyst, 2013,138, 1988-1994

Label-free, disposable fiber-optic biosensors for DNA hybridization detection

M. Yin, C. Wu, L. Shao, W. K. E. Chan, A. P. Zhang, C. Lu and H. Tam, Analyst, 2013, 138, 1988 DOI: 10.1039/C3AN36791F

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