Issue 14, 2013
From the journal:

Analytical Methods

Time-resolved fluoroimmunoassay for quantitative determination of thiacloprid in agricultural samples†

Zhenjiang Liu,a   Xu Yan,a   Xiude Huaa  and  Minghua Wang*a  

* Corresponding authors

a Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Jiangsu Key Laboratory of Pesticide Science, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, P. R. China
E-mail: wangmha@njau.edu.cn
Fax: +86 25 84395479
Tel: +86 25 84395479

Abstract

A fast and sensitive indirect competitive time-resolved fluoroimmunoassay (TRFIA) for thiacloprid is developed. Europium-labeled goat anti-rabbit antibody was used as the tracer. Under optimal conditions, the TRFIA showed a 50% inhibition concentration (IC50) value of 2 μg L−1 with a limit of detection (LOD, IC10) of 1.9 ng L−1. The recoveries from agricultural samples, including water, soil, pear and tomato, were in the range from 83.4% to 121% with a relative standard deviation of 2.40–11.3%. The TRFIA results for real soil samples were confirmed by high performance liquid chromatography with a high correlation coefficient of 0.9854. This analytical method demonstrates good sensitivity and stability, and its potential for practical applications.

Graphical abstract: Time-resolved fluoroimmunoassay for quantitative determination of thiacloprid in agricultural samples

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Article information

DOI
https://doi.org/10.1039/C3AY00033H
Article type
Paper
Submitted
05 Jan 2013
Accepted
02 May 2013
First published
08 May 2013

Anal. Methods, 2013,5, 3572-3576

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Time-resolved fluoroimmunoassay for quantitative determination of thiacloprid in agricultural samples

Z. Liu, X. Yan, X. Hua and M. Wang, Anal. Methods, 2013, 5, 3572 DOI: 10.1039/C3AY00033H

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