Spectral method determination of kanamycin sulfate using both gold nanoparticles and quantum dots

Abstract

Two novel methods for the determination of kanamycin sulfate (Kan) have been developed. The first method was based on the enhanced fluorescence of thioglycolic acid-capped CdTe quantum dots by Kan in aqueous solutions. The second method was a simple colorimetric method to detect trace amounts of Kan with unmodified gold nanoparticles as the sensing probe. Under optimal conditions, the relative fluorescence intensity was linearly proportional to the concentration of Kan between 1.80 × 10⁻² μg mL⁻¹ and 8.00 × 10⁻¹ μg mL⁻¹. The linear ranges of the colorimetric sensor for Kan were from 3.75 × 10⁻³ μg mL⁻¹ to 4.25 × 10⁻² μg mL⁻¹ and from 4.40 × 10⁻² μg mL⁻¹ to 9.88 × 10⁻² μg mL⁻¹, respectively. Comparisons between the two methods were made. Both methods were used for rapid detection of Kan in real samples with satisfactory results.