Issue 5, 2013
From the journal:

Chemical Communications

Branched DNA nanostructures efficiently stabilised and monitored by novel pyrene–perylene 2′-α-l-amino-LNA FRET pairs†‡

I. Kira Astakhova,*a   T. Santhosh Kumar,a   Meghan A. Campbell,a   Alexey V. Ustinov,b   Vladimir A. Korshunb  and  Jesper Wengel*a  

* Corresponding authors

a Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, DK-5230 Odense M, Denmark
E-mail: ias@sdu.dk, jwe@sdu.dk
Fax: +45 6615 8780
Tel: +45 6550 2510

b Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
Fax: +7 499 7246715
Tel: +7 499 7246715

Abstract

Novel pyrene–perylene α-L-LNA FRET pairs described herein effectively detect assembly of 2- and 3-way branched DNA nanostructures prepared by postsynthetic microwave-assisted CuAAC click chemistry. The fluorescent signalling of assembly by internally positioned FRET pairs is achieved with low to no fluorescence background signal, remarkably low limit of target detection values and stabilization of the resulting nanostructures.

Graphical abstract: Branched DNA nanostructures efficiently stabilised and monitored by novel pyrene–perylene 2′-α-l-amino-LNA FRET pairs

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Article information

DOI
https://doi.org/10.1039/C2CC37547H
Article type
Communication
Submitted
16 Oct 2012
Accepted
19 Nov 2012
First published
20 Nov 2012

Chem. Commun., 2013,49, 511-513

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Branched DNA nanostructures efficiently stabilised and monitored by novel pyrene–perylene 2′-α-L-amino-LNA FRET pairs

I. K. Astakhova, T. Santhosh Kumar, M. A. Campbell, A. V. Ustinov, V. A. Korshun and J. Wengel, Chem. Commun., 2013, 49, 511 DOI: 10.1039/C2CC37547H

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