A universal and multiplex kinase assay using γ-[18O4]-ATP

Chuan Fu; Xueyun Zheng; Yao Jiang; Yan Liu; Pengxiang Xu; Zhiping Zeng; Rihe Liu; Yufen Zhao

doi:10.1039/C3CC38467E

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A universal and multiplex kinase assay using γ-[¹⁸O₄]-ATP†

Chuan Fu,^a Xueyun Zheng,^a Yao Jiang,^a Yan Liu,^a Pengxiang Xu,^a Zhiping Zeng,^a Rihe Liu^b and Yufen Zhao*^a

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* Corresponding authors

^a Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, P.R.China
E-mail: yfzhao@xmu.edu.cn

^b Eshelman School of Pharmacy and Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7568, USA

Abstract

We have synthesized γ-[¹⁸O₄]-ATP and used it to develop a non-radioactive and multiplex method. Significantly, this novel approach can be applied to any kinases without using a purified enzyme or a fluorescent substrate. Using this approach, the effectiveness and specificity of inhibitors on several kinases could be readily determined.

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Article information

DOI: https://doi.org/10.1039/C3CC38467E
Article type: Communication
Submitted: 25 Nov 2012
Accepted: 16 Jan 2013
First published: 18 Jan 2013

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Chem. Commun., 2013,49, 2795-2797

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A universal and multiplex kinase assay using γ-[¹⁸O₄]-ATP

C. Fu, X. Zheng, Y. Jiang, Y. Liu, P. Xu, Z. Zeng, R. Liu and Y. Zhao, Chem. Commun., 2013, 49, 2795 DOI: 10.1039/C3CC38467E

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