Issue 18, 2013
From the journal:

Chemical Communications

Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein†

Shota Morimoto,a   Takenori Tomohiro,*a   Nobuyuki Maruyamab  and  Yasumaru Hatanaka*a  

* Corresponding authors

a Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan
E-mail: ttomo@pha.u-toyama.ac.jp, yasu@pha.u-toyama.ac.jp
Fax: +81 76-434-5063
Tel: +81 76-434-7516

b Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan

Abstract

A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.

Graphical abstract: Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein

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Article information

DOI
https://doi.org/10.1039/C3CC38594A
Article type
Communication
Submitted
30 Nov 2012
Accepted
11 Jan 2013
First published
15 Jan 2013

Chem. Commun., 2013,49, 1811-1813

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Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein

S. Morimoto, T. Tomohiro, N. Maruyama and Y. Hatanaka, Chem. Commun., 2013, 49, 1811 DOI: 10.1039/C3CC38594A

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