Issue 17, 2013
From the journal:

Chemical Communications

High density DNA loading on the M13 bacteriophage provides access to colorimetric and fluorescent protein microarray biosensors†

Dylan W. Domaille,a   Ju Hun Leeb  and  Jennifer N. Cha*a  

* Corresponding authors

a Department of Chemical and Biological Engineering, University of Colorado, Boulder, Boulder, CO, USA
E-mail: Jennifer.Cha@colorado.edu

b Materials Science and Engineering Program, University of California, San Diego, San Diego, CA, USA

Abstract

We report the synthesis, characterization, and protein sensing capabilities of M13 bacteriophage–DNA bioconjugates. DNA oligonucleotides were conjugated to M13 through acyl hydrazone linkages. In one case, DNAzymes retained their catalytic ability when anchored to the virus coat, and in a separate study, the dynamic nature of the hydrazone allowed for liberation of DNA from the phage under mild conditions.

Graphical abstract: High density DNA loading on the M13 bacteriophage provides access to colorimetric and fluorescent protein microarray biosensors

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C3CC38871A
Article type
Communication
Submitted
11 Dec 2012
Accepted
10 Jan 2013
First published
11 Jan 2013

Chem. Commun., 2013,49, 1759-1761

Permissions

Request permissions

High density DNA loading on the M13 bacteriophage provides access to colorimetric and fluorescent protein microarray biosensors

D. W. Domaille, J. H. Lee and J. N. Cha, Chem. Commun., 2013, 49, 1759 DOI: 10.1039/C3CC38871A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements