Issue 41, 2013
From the journal:

Chemical Communications

P450 BM3 crystal structures reveal the role of the charged surface residue Lys/Arg184 in inversion of enantioselective styrene epoxidation†

Aamir Shehzad,‡a   Saravanan Panneerselvam,‡b   Marina Linow,c   Marco Bocola,a   Danilo Roccatano,d   Jochen Mueller-Dieckmann,e   Matthias Wilmannsf  and  Ulrich Schwaneberg*a  

* Corresponding authors

a Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany
E-mail: u.schwaneberg@biotec.rwth-aachen.de
Fax: +49 2418022387
Tel: +49 2418024170

b HASYLAB, DESY, Notkestraße 85, 22603 Hamburg, Germany

c Molzym GmbH & Co. KG, Mary-Astell-Str. 10, Bremen, Germany

d Jacobs University Bremen, 28759 Bremen, Germany

e Biocenter Klein Flottbek, University of Hamburg, Ohnhorststr. 18, 22609 Hamburg, Germany

f European Molecular Biology Laboratory – Hamburg, c/o DESY, Hamburg, Germany

Abstract

Solved crystal structures of P450 BM3 variants in complex with styrene provide on the molecular level a first explanation of how a positively charged surface residue inverts the enantiopreference of styrene epoxidation. The obtained insights into productive and non-productive styrene binding modes deepened our understanding of enantioselective epoxidation with P450 BM3.

Graphical abstract: P450 BM3 crystal structures reveal the role of the charged surface residue Lys/Arg184 in inversion of enantioselective styrene epoxidation

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Article information

DOI
https://doi.org/10.1039/C3CC39076D
Article type
Communication
Submitted
19 Dec 2012
Accepted
04 Apr 2013
First published
05 Apr 2013

Chem. Commun., 2013,49, 4694-4696

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P450 BM3 crystal structures reveal the role of the charged surface residue Lys/Arg184 in inversion of enantioselective styrene epoxidation

A. Shehzad, S. Panneerselvam, M. Linow, M. Bocola, D. Roccatano, J. Mueller-Dieckmann, M. Wilmanns and U. Schwaneberg, Chem. Commun., 2013, 49, 4694 DOI: 10.1039/C3CC39076D

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