Issue 48, 2013
From the journal:

Chemical Communications

A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres†

Aarthi Chandrasekaran,*ab   Kai Deng,ab   Chung-Yan Koh,b   Taichi Takasuka,c   Lai F. Bergeman,c   Brian G. Fox,c   Paul D. Adamsad  and  Anup K. Singh*ab  

* Corresponding authors

a Technology Division, Joint BioEnergy Institute, Emeryville, CA, USA
E-mail: achandrasekaran@lbl.gov, aksingh@sandia.gov
Fax: +1-925-294-3020
Tel: +1-925-294-1260

b Biotechnology and Bioengineering Department, Sandia National Laboratories, Livermore, CA, USA

c Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, WI, USA

d Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA

Abstract

We describe a simple, multiplexed assay that integrates glycan synthesis, bioconjugation to microspheres, fluorescent chemical/biochemical detection and multiparameter flow cytometric analysis to screen activities of different families of carbohydrate-active enzymes.

Graphical abstract: A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C3CC39155H
Article type
Communication
Submitted
21 Dec 2012
Accepted
11 Mar 2013
First published
12 Mar 2013

Chem. Commun., 2013,49, 5441-5443

Permissions

Request permissions

A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres

A. Chandrasekaran, K. Deng, C. Koh, T. Takasuka, L. F. Bergeman, B. G. Fox, P. D. Adams and A. K. Singh, Chem. Commun., 2013, 49, 5441 DOI: 10.1039/C3CC39155H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements