Issue 36, 2013
From the journal:

Chemical Communications

Functional assembly of a multi-enzyme methanol oxidation cascade on a surface-displayed trifunctional scaffold for enhanced NADH production†

Fang Liu,a   Scott Bantab  and  Wilfred Chen*a  

* Corresponding authors

a Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA
E-mail: wilfred@udel.edu
Fax: +1 30 2831 1048
Tel: +1 30 2831 6327

b Department of Chemical Engineering, Columbia University, New York, NY 10027, USA

Abstract

We report a simple and low-cost strategy that allows the sequential and site-specific assembly of a dehydrogenase-based multi-enzyme cascade for methanol oxidation on the yeast surface using the high-affinity interactions between three orthogonal cohesin–dockerin pairs. The multi-enzyme cascade showed 5 times higher NADH production rate than the non-complexed enzyme mixture, a result of efficient substrate channeling.

Graphical abstract: Functional assembly of a multi-enzyme methanol oxidation cascade on a surface-displayed trifunctional scaffold for enhanced NADH production

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Article information

DOI
https://doi.org/10.1039/C3CC40454D
Article type
Communication
Submitted
18 Jan 2013
Accepted
20 Mar 2013
First published
21 Mar 2013

Chem. Commun., 2013,49, 3766-3768

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Functional assembly of a multi-enzyme methanol oxidation cascade on a surface-displayed trifunctional scaffold for enhanced NADH production

F. Liu, S. Banta and W. Chen, Chem. Commun., 2013, 49, 3766 DOI: 10.1039/C3CC40454D

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