Exploring the differences between mouse mAβ1–42 and human hAβ1–42 for Alzheimer's disease related properties and neuronal cytotoxicity

Abstract

The differences between mouse mAβ_1–42 and human hAβ_1–42, explored using CD and fluorescence spectroscopy, transmission electron microscopy, ROS fluorescent assay, and neuronal cell viability, revealed that mAβ_1–42 as a three-site mutant (R5G, Y10F and H13R) of hAβ_1–42 altered the metal (copper and zinc) binding sites, reduced the proneness to form β-sheet structures and aggregated fibrils, alleviated the generation of ROS, and decreased the cytotoxicity, in contrast to hAβ_1–42.