Issue 42, 2013
From the journal:

Chemical Communications

A simple new competition assay for heparin binding in serum applied to multivalent PAMAM dendrimers†

Stephen M. Bromfield,a   Paola Posocco,b   Maurizio Fermeglia,b   Sabrina Pricl,bc   Julián Rodríguez-Lópezd  and  David K. Smith*a  

* Corresponding authors

a Department of Chemistry, University of York, Heslington, York, UK
E-mail: david.smith@york.ac.uk

b Molecular Simulation Engineering (MOSE) Laboratory, Department of Engineering and Architecture (DEA), University of Trieste, 34127 Trieste, Italy

c National Interuniversity Consortium for Material Science and Technology (INSTM), Research Unit MOSE-DEA, University of Trieste, 34127 Trieste, Italy

d Área de Química Orgánica, Facultad de Ciencias y Tecnologías Químicas, Universidad de Castilla la Mancha, 13071 Ciudad Real, Spain

Abstract

We report a competition assay using our recently reported dye Mallard Blue, which allows us to identify synthetic heparin binders in competitive media, including human serum – using this we gain insight into the ability of PAMAM dendrimers to bind heparin, with the interesting result that low-generation G2-PAMAM is the preferred heparin binder.

Graphical abstract: A simple new competition assay for heparin binding in serum applied to multivalent PAMAM dendrimers

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Article information

DOI
https://doi.org/10.1039/C3CC41251B
Article type
Communication
Submitted
17 Feb 2013
Accepted
11 Apr 2013
First published
18 Apr 2013

Chem. Commun., 2013,49, 4830-4832

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A simple new competition assay for heparin binding in serum applied to multivalent PAMAM dendrimers

S. M. Bromfield, P. Posocco, M. Fermeglia, S. Pricl, J. Rodríguez-López and D. K. Smith, Chem. Commun., 2013, 49, 4830 DOI: 10.1039/C3CC41251B

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