Issue 57, 2013
From the journal:

Chemical Communications

A novel array format for monitoring cellular uptake using a photo-cleavable linker for peptide release†

Kenji Usui,‡*ab   Takuya Kikuchi,‡a   Kin-ya Tomizaki,c   Takashi Kakiyamaa  and  Hisakazu Mihara*a  

* Corresponding authors

a Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho 4259 B-40, Midori-ku, Yokohama 226-8501, Japan
E-mail: hmihara@bio.titech.ac.jp
Fax: +81 45 924 5833
Tel: +81 45 924 5756

b FIRST (Faculty of Frontiers of Innovative Research in Science and Technology) and FIBER (Frontier Institute for Biomolecular Engineering Research), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan
E-mail: kusui@center.konan-u.ac.jp
Fax: +81 78 303 1495
Tel: +81 78 303 1418

c Innovative Materials and Processing Research Center and Department of Materials Chemistry, Ryukoku University, 1-5 Yokotani, Seta Oe-cho, Otsu 520-2194, Japan

Abstract

We developed a novel peptide array format incorporating a photo-cleavable linker for monitoring cellular uptake. Model peptides were successfully immobilised via the photo-cleavable linker onto conventional plates and could be released spatiotemporally using UV irradiation. Incorporation of confocal microscopy allowed for detailed real-time monitoring of cellular internalisation of peptides.

Graphical abstract: A novel array format for monitoring cellular uptake using a photo-cleavable linker for peptide release

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Article information

DOI
https://doi.org/10.1039/C3CC41632A
Article type
Communication
Submitted
04 Mar 2013
Accepted
22 May 2013
First published
10 Jun 2013

Chem. Commun., 2013,49, 6394-6396

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A novel array format for monitoring cellular uptake using a photo-cleavable linker for peptide release

K. Usui, T. Kikuchi, K. Tomizaki, T. Kakiyama and H. Mihara, Chem. Commun., 2013, 49, 6394 DOI: 10.1039/C3CC41632A

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