Issue 47, 2013
From the journal:

Chemical Communications

A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells†

Brian K. McMahon,a   Robert Pala  and  David Parker*a  

* Corresponding authors

a Department of Chemistry, Durham University, South Road, Durham, UK
E-mail: david.parker@dur.ac.uk

Abstract

A ratiometric EuIII complex has been developed that localises selectively within the endoplasmic reticulum of living cells. Careful calibration, using a time-gated spectral imaging microscope, allows the intensity ratio of emission bands and the variation of excited state lifetime to be used for pH determination, with a pKa of 7.15.

Graphical abstract: A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells

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Article information

DOI
https://doi.org/10.1039/C3CC42308E
Article type
Communication
Submitted
29 Mar 2013
Accepted
30 Apr 2013
First published
01 May 2013

Chem. Commun., 2013,49, 5363-5365

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A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells

B. K. McMahon, R. Pal and D. Parker, Chem. Commun., 2013, 49, 5363 DOI: 10.1039/C3CC42308E

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