Issue 77, 2013
From the journal:

Chemical Communications

Optical detection of epigenetic marks: sensitive quantification and direct imaging of individual hydroxymethylcytosine bases†

Yael Michaeli,‡a   Tamar Shahal,‡a   Dmitry Torchinsky,a   Assaf Grunwald,a   Rony Hocha  and  Yuval Ebenstein*a  

* Corresponding authors

a Raymond and Beverly Sackler Faculty of Exact Sciences, School of Chemistry, Tel Aviv University, Tel Aviv, Israel
E-mail: uv@post.tau.ac.il
Tel: +972 3 640 8901

Abstract

Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.

Graphical abstract: Optical detection of epigenetic marks: sensitive quantification and direct imaging of individual hydroxymethylcytosine bases

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Article information

DOI
https://doi.org/10.1039/C3CC42543F
Article type
Communication
Submitted
08 Apr 2013
Accepted
29 May 2013
First published
29 May 2013

Chem. Commun., 2013,49, 8599-8601

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Optical detection of epigenetic marks: sensitive quantification and direct imaging of individual hydroxymethylcytosine bases

Y. Michaeli, T. Shahal, D. Torchinsky, A. Grunwald, R. Hoch and Y. Ebenstein, Chem. Commun., 2013, 49, 8599 DOI: 10.1039/C3CC42543F

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