Rapid dissolution of DNA in a novel bio-based ionic liquid with long-term structural and chemical stability: successful recycling of the ionic liquid for reuse in the process

Abstract

DNA from salmon testes was solubilised in two bio-based ionic liquids up to 3.5% w/w in 6 h. No structural degradation of the molecule was observed for the sample solubilised in choline-indole-3-acetate (chol-IAA). However, the molecule was found to be denatured in choline-indole-3-butyrate (chol-IBA). The structural and chemical stability of the DNA molecules after six months of storage in the former was established. Further recyclability of the ionic liquid with very high yield (90–95%) for consecutive reuse in the redissolution of DNA was demonstrated.