Issue 76, 2013

Real-time monitoring of DNA hybridization kinetics on living cell surfaces

Abstract

We determined hybridization rates of DNA probes bound to the surfaces of a human cell, and compared the rates with those determined in solution. The rates were slower on the cell surface than in solution-phase. The position of the nucleation site but not the location of the formed duplex relative to the cell surface adversely affected the hybridization kinetics.

Graphical abstract: Real-time monitoring of DNA hybridization kinetics on living cell surfaces

Supplementary files

Article information

Article type
Communication
Submitted
23 Apr 2013
Accepted
30 May 2013
First published
30 May 2013

Chem. Commun., 2013,49, 8444-8446

Real-time monitoring of DNA hybridization kinetics on living cell surfaces

A. B. Rode, T. Endoh, H. Tateishi-Karimata, S. Takahashi and N. Sugimoto, Chem. Commun., 2013, 49, 8444 DOI: 10.1039/C3CC42990C

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