Issue 60, 2013

An artificial restriction DNA cutter for site-selective gene insertion in human cells

Abstract

With the use of a chemistry-based artificial restriction DNA cutter (combination of Ce(IV)–EDTA and a pair of pcPNA), both an antibiotic-resistance gene and a fluorescent reporter protein gene were incorporated into the targeted site through homologous recombination in human cells.

Graphical abstract: An artificial restriction DNA cutter for site-selective gene insertion in human cells

Supplementary files

Article information

Article type
Communication
Submitted
02 May 2013
Accepted
03 Jun 2013
First published
04 Jun 2013

Chem. Commun., 2013,49, 6764-6766

An artificial restriction DNA cutter for site-selective gene insertion in human cells

K. Ito, N. Shigi and M. Komiyama, Chem. Commun., 2013, 49, 6764 DOI: 10.1039/C3CC43261K

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