Issue 78, 2013

Ultrasensitive detection of small molecule–protein interaction via terminal protection of small molecule linked DNA and Exo III-aided DNA recycling amplification

Abstract

Terminal protection of small molecule linked DNA assays amplified by Exo III-aided DNA recycling is developed for the detection of streptavidin–biotin interaction, with a detection limit of 0.8 fM streptavidin in the “turn-on” state, indicating that the proposed method is an ultrasensitive platform for the detection of small molecule–protein interactions.

Graphical abstract: Ultrasensitive detection of small molecule–protein interaction via terminal protection of small molecule linked DNA and Exo III-aided DNA recycling amplification

Supplementary files

Article information

Article type
Communication
Submitted
14 May 2013
Accepted
23 Jul 2013
First published
23 Jul 2013

Chem. Commun., 2013,49, 8854-8856

Ultrasensitive detection of small molecule–protein interaction via terminal protection of small molecule linked DNA and Exo III-aided DNA recycling amplification

G. Zhou, X. Zhang, X. Ji and Z. He, Chem. Commun., 2013, 49, 8854 DOI: 10.1039/C3CC43621G

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