Issue 86, 2013

A glyco-gold nanoparticle based assay for α-2,8-polysialyltransferase from Neisseria meningitidis

Abstract

We designed a novel strategy for sensitively detecting the activity of α-2,8-polysialyltransferase (PST) by a combination of ganglioside GD3 functionalized gold nanoparticles and inactive endosialidase. We anticipate that this new method will facilitate the search for PST inhibitors as well as for improved mutant forms of PST in directed evolution experiments.

Graphical abstract: A glyco-gold nanoparticle based assay for α-2,8-polysialyltransferase from Neisseria meningitidis

Supplementary files

Article information

Article type
Communication
Submitted
09 Jul 2013
Accepted
03 Sep 2013
First published
09 Sep 2013

Chem. Commun., 2013,49, 10166-10168

A glyco-gold nanoparticle based assay for α-2,8-polysialyltransferase from Neisseria meningitidis

C. Yu, L. Huang, D. H. Kwan, W. W. Wakarchuk, S. G. Withers and C. Lin, Chem. Commun., 2013, 49, 10166 DOI: 10.1039/C3CC45147J

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