Issue 86, 2013
From the journal:

Chemical Communications

DNA analysis based on toehold-mediated strand displacement on graphene oxide†

Takaaki Miyahata,a   Yusuke Kitamura,*ab   Akika Futamura,a   Hirotaka Matsuura,a   Kazuto Hatakeyama,a   Michio Koinuma,ab   Yasumichi Matsumotoab  and  Toshihiro Ihara*ab  

* Corresponding authors

a Department of Applied Chemistry and Biochemistry, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, Japan
E-mail: ykita@kumamoto-u.ac.jp, toshi@chem.kumamoto-u.ac.jp
Fax: +81 96 342 3873
Tel: +81 96 342 3873

b CREST, Japan Science and Technology Agency, 7 Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan

Abstract

Fluorescent dye-labeled probe DNA was immobilized on fluorescence-quenching graphene oxide (GO) through a capture DNA. When targets were added, the probes were released from the GO through toehold-mediated strand exchange. Higher emission recovery and more signal contrast were achieved relative to conventional methods that are based on direct adsorption of probes.

Graphical abstract: DNA analysis based on toehold-mediated strand displacement on graphene oxide

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Article information

DOI
https://doi.org/10.1039/C3CC45531A
Article type
Communication
Submitted
22 Jul 2013
Accepted
04 Sep 2013
First published
05 Sep 2013

Chem. Commun., 2013,49, 10139-10141

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DNA analysis based on toehold-mediated strand displacement on graphene oxide

T. Miyahata, Y. Kitamura, A. Futamura, H. Matsuura, K. Hatakeyama, M. Koinuma, Y. Matsumoto and T. Ihara, Chem. Commun., 2013, 49, 10139 DOI: 10.1039/C3CC45531A

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