Issue 100, 2013
From the journal:

Chemical Communications

High-throughput tandem-microwell assay identifies inhibitors of the hydrogen sulfide signaling pathway†

Yueyang Zhou,‡a   Jing Yu,‡b   Xiling Lei,a   Jianying Wu,c   Qi Niu,a   Yixuan Zhang,c   Hong Liu,d   Philipp Christen,e   Heinz Gehringe  and  Fang Wu*a  

* Corresponding authors

a Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China
E-mail: fang.wu@sjtu.edu.cn

b State Key Laboratory of Microbial Metabolism & School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China

c School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, China

d CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China

e Department of Biochemistry, University of Zurich, 8057 Zurich, Switzerland

Abstract

We report a high-throughput assay for H2S-producing enzymes, which is based on a newly designed tandem-well plate. Screening of 21 599 agents identified several potent inhibitors of cystathionine β-synthase and cystathionine γ-lyase, the two key enzymes generating H2S in mammals, with IC50 values in the low two-digit micromolar range.

Graphical abstract: High-throughput tandem-microwell assay identifies inhibitors of the hydrogen sulfide signaling pathway

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Article information

DOI
https://doi.org/10.1039/C3CC46719H
Article type
Communication
Submitted
03 Sep 2013
Accepted
24 Oct 2013
First published
24 Oct 2013

Chem. Commun., 2013,49, 11782-11784

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High-throughput tandem-microwell assay identifies inhibitors of the hydrogen sulfide signaling pathway

Y. Zhou, J. Yu, X. Lei, J. Wu, Q. Niu, Y. Zhang, H. Liu, P. Christen, H. Gehring and F. Wu, Chem. Commun., 2013, 49, 11782 DOI: 10.1039/C3CC46719H

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