PET-dependent fluorescence sensing of enzyme reactions using the large and tunable pKa shift of aliphatic amines

Abstract

A fluorescence sensing system exploiting the large and finely tunable pK_a shift of aliphatic amines was developed. The amine-containing fluorescent probes with distinct pK_a values in a wide pH range were successfully applied to detect a variety of enzyme reactions with a large and real-time fluorescence enhancement.