Issue 10, 2013
From the journal:

Physical Chemistry Chemical Physics

Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation†

Igor Tkach,a   Soraya Pornsuwan,a   Claudia Höbartner,b   Falk Wachowius,b   Snorri Th. Sigurdsson,c   Tatiana Y. Baranova,d   Ulf Diederichsen,d   Giuseppe Sicolia  and  Marina Bennati*ad  

* Corresponding authors

a Research Group EPR Spectroscopy, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
E-mail: marina.bennati@mpibpc.mpg.de
Fax: +49 551 201 1383
Tel: +49 551 201 1911

b Research Group Nucleic Acid Chemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany

c University of Iceland, Department of Chemistry, Science Institute, Dunhaga 3, Reykjavik, Iceland

d Institute of Organic and Biomolecular Chemistry, Georg-August University of Göttingen, Tammannstr. 2, Göttingen, Germany

Abstract

Pulsed electron–electron double resonance (PELDOR, also known as DEER) has become a method of choice to measure distances in biomolecules. In this work we show how the performance of the method can be improved at high EPR frequencies (94 GHz) using variable dual frequency irradiation in a dual mode cavity in order to obtain enhanced resolution toward orientation selection. Dipolar evolution traces of a representative RNA duplex and an α-helical peptide were analysed in terms of possible bi-radical structures by considering the inherent ambiguity of symmetry-related solutions.

Graphical abstract: Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C3CP44415E
Article type
Communication
Submitted
08 Dec 2012
Accepted
15 Jan 2013
First published
17 Jan 2013

Phys. Chem. Chem. Phys., 2013,15, 3433-3437

Permissions

Request permissions

Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation

I. Tkach, S. Pornsuwan, C. Höbartner, F. Wachowius, S. Th. Sigurdsson, T. Y. Baranova, U. Diederichsen, G. Sicoli and M. Bennati, Phys. Chem. Chem. Phys., 2013, 15, 3433 DOI: 10.1039/C3CP44415E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements