Calciomics: integrative studies of Ca2+-binding proteins and their interactomes in biological systems

Abstract

Calcium ion (Ca²⁺), the fifth most common chemical element in the earth's crust, represents the most abundant mineral in the human body. By binding to a myriad of proteins distributed in different cellular organelles, Ca²⁺ impacts nearly every aspect of cellular life. In prokaryotes, Ca²⁺ plays an important role in bacterial movement, chemotaxis, survival reactions and sporulation. In eukaryotes, Ca²⁺ has been chosen through evolution to function as a universal and versatile intracellular signal. Viruses, as obligate intracellular parasites, also develop smart strategies to manipulate the host Ca²⁺ signaling machinery to benefit their own life cycles. This review focuses on recent advances in applying both bioinformatic and experimental approaches to predict and validate Ca²⁺-binding proteins and their interactomes in biological systems on a genome-wide scale (termed “calciomics”). Calmodulin is used as an example of Ca²⁺-binding protein (CaBP) to demonstrate the role of CaBPs on the regulation of biological functions. This review is anticipated to rekindle interest in investigating Ca²⁺-binding proteins and Ca²⁺-modulated functions at the systems level in the post-genomic era.