Single KTP nanocrystals as second-harmonic generation biolabels in cortical neurons

Ludovic Mayer; Abdallah Slablab; Géraldine Dantelle; Vincent Jacques; Aude-Marie Lepagnol-Bestel; Sandrine Perruchas; Piernicola Spinicelli; Alice Thomas; Dominique Chauvat; Michel Simonneau; Thierry Gacoin; Jean-François Roch

doi:10.1039/C3NR01251D

Single KTP nanocrystals as second-harmonic generation biolabels in cortical neurons†

Ludovic Mayer,^a Abdallah Slablab,^b Géraldine Dantelle,^a Vincent Jacques,^bc Aude-Marie Lepagnol-Bestel,^d Sandrine Perruchas,^a Piernicola Spinicelli,^b Alice Thomas,^e Dominique Chauvat,^b Michel Simonneau,^e Thierry Gacoin*^a and Jean-François Roch*^c

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* Corresponding authors

^a Laboratoire de Physique de la Matière Condensée – Ecole Polytechnique-CNRS, UMR 7643, 91128 Palaiseau Cedex, France
E-mail: thierry.gacoin@polytechnique.edu

^b Laboratoire de Photonique Quantique et Moléculaire – ENS Cachan & CNRS, UMR 8537, 94235 Cachan, France

^c Laboratoire Aimé Cotton, CNRS, Université Paris-Sud & ENS Cachan, 91405 Orsay, France
E-mail: jean-francois.roch@ens-cachan.fr

^d CEA/CNG, Laboratoire Génomique Fonctionnelle, 91057 Evry, France

^e INSERM 894, Centre Psychiatrie & Neurosciences de l’INSERM, 75014 Paris, France

Abstract

We report an efficient colloidal synthesis of KTiOPO₄ (KTP) nanocrystals with excellent crystallinity and the direct observation of optical second-harmonic generation (SHG) from discrete KTP nanocrystals in neurons cultured from mammalian brain cortex. Direct internalization and monitoring of these nanoparticles was successfully achieved without limitations from cytotoxicity, bleaching and blinking emission.

Nanoscale

Single KTP nanocrystals as second-harmonic generation biolabels in cortical neurons†

Abstract

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Single KTP nanocrystals as second-harmonic generation biolabels in cortical neurons

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