A novel ratiometric emission probe for Ca2+ in living cells

Qiaoling Liu; Wei Bian; Heping Shi; Li Fan; Shaomin Shuang; Chuan Dong; Martin M. F. Choi

doi:10.1039/C2OB26888D

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A novel ratiometric emission probe for Ca²⁺ in living cells†

Qiaoling Liu,^ab Wei Bian,^a Heping Shi,^a Li Fan,^a Shaomin Shuang,^a Chuan Dong*^a and Martin M. F. Choi*^c

Author affiliations

* Corresponding authors

^a Research Center of Environmental Science and Engineering, and School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, P. R. China
E-mail: dc@sxu.edu.cn
Fax: +86-0351-7018613
Tel: +86-0351-7018613

^b Department of Chemistry, Taiyuan Normal University, Taiyuan 030031, P. R. China

^c Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong SAR, P. R. China
E-mail: mfchoi@hkbu.edu.hk
Fax: +852-34117348
Tel: +852-34117839

Abstract

A ratiometric fluorescent probe for Ca²⁺ based on 1,3,4-oxadiazole derivative has been designed and developed. The probe exhibits a large Stokes shift of 202 nm and a highly selective ratiometric emission response (490/582 nm) to Ca²⁺ over other metal cations. Additionally, the probe can readily reveal the changes of intracellular Ca²⁺ concentration in living human umbilical vein endothelial cells.

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Article information

DOI: https://doi.org/10.1039/C2OB26888D
Article type: Paper
Submitted: 25 Sep 2012
Accepted: 06 Nov 2012
First published: 07 Nov 2012

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Org. Biomol. Chem., 2013,11, 503-508

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A novel ratiometric emission probe for Ca²⁺ in living cells

Q. Liu, W. Bian, H. Shi, L. Fan, S. Shuang, C. Dong and M. M. F. Choi, Org. Biomol. Chem., 2013, 11, 503 DOI: 10.1039/C2OB26888D

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Organic & Biomolecular Chemistry