Issue 26, 2013
From the journal:

Organic & Biomolecular Chemistry

Highly specific, multi-branched fluorescent reporters for analysis of human neutrophil elastase†

Nicolaos Avlonitis,‡ab   Manuelle Debunne,‡b   Tashfeen Aslam,a   Neil McDonald,b   Chris Haslett,b   Kevin Dhaliwalb  and  Mark Bradley*a  

* Corresponding authors

a EaStChem, School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh, UK
E-mail: mark.bradley@ed.ac.uk

b MRC/Centre of Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, UK

Abstract

Human neutrophil elastase (HNE) is a serine protease implicated in the pathogenesis of acute and chronic inflammatory disease. Here a series of, internally quenched, single fluorophore fluorescent reporters were synthesised that allowed the rapid, highly specific and sensitive analysis of HNE activity over closely related proteases.

Graphical abstract: Highly specific, multi-branched fluorescent reporters for analysis of human neutrophil elastase

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Article information

DOI
https://doi.org/10.1039/C3OB40212F
Article type
Paper
Submitted
30 Jan 2013
Accepted
29 Apr 2013
First published
01 May 2013

Org. Biomol. Chem., 2013,11, 4414-4418

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Highly specific, multi-branched fluorescent reporters for analysis of human neutrophil elastase

N. Avlonitis, M. Debunne, T. Aslam, N. McDonald, C. Haslett, K. Dhaliwal and M. Bradley, Org. Biomol. Chem., 2013, 11, 4414 DOI: 10.1039/C3OB40212F

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